OpenCRISPR-1
#1 in Science & Bio AIProfluent · v1 · since 22. April 2024 (initialer Open-Source-Release); Peer-Review-Publikation in Nature am 30. Juli 2025 · 9× · last seen Jul 29, 2026
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OpenCRISPR-1 is a fully AI-generated genome editor from Berkeley startup Profluent Bio, consisting of a Cas9-like protein and matching guide RNA, entirely designed using Profluent's large language models. The protein sequence is hundreds of mutations away from natural SpCas9 yet achieves comparable editing activity with roughly 95% fewer off-target cuts and lower immunogenicity. The system was released as a preprint and open-source release in April 2024 and peer-reviewed published in Nature in July 2025; sequences are freely available via GitHub and Addgene.
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01.05.30.07.
Features
| Compliance/Certification | Peer-reviewed publication in Nature (2025); ethics license prohibits germline editing and unethical applications |
| Deployment Model | Open-source sequence data (no hosted service); use requires lab synthesis/plasmid transfection, e.g., via Addgene plasmids |
| Use Case Scope | Genome editing as drop-in replacement for SpCas9 protocols; compatible with base, prime, and epigenome editing |
| Integrations | Compatible with canonical SpCas9 gRNAs; usable in deactivated or nickase format |
| License | Free for research and commercial use, but requires a mandatory ethics license agreement (prohibits germline editing and other 'Restricted Purposes') |
| Platform | Protein sequence + gRNA, available as FASTA files on GitHub and as plasmids on Addgene |
| Price | Free for ethical research and commercial use, subject to signing a simple license agreement |
| Release Date | April 22, 2024 (open-source release), Nature publication July 30, 2025 |